Raman microspectroscopy is a laboratory process to produce molecular fingerprints of materials and biological specimens. Nevertheless, to date, fluorescence has interfered with useful software of this method and limited its use. Now Gordon Taylor, a Professor in the School of Marine and Atmospheric Sciences at Stony Brook University, and colleagues have devised a photochemical process that suppresses fluorescence in sample preparation. This new approach may open the door to more robust and extremely resolved investigations of chemical distributions within individual cells. Their findings are printed in Scientific Reports.
Characterizing cell-to-cell and intracellular versions in biochemistry are essential to mechanistic understandings in research that covers a large area, including cancer, human development, cell biology, antibiotics exploration, and environmental biology. Laser-based Raman microspectroscopy is among only some instruments that scientists can use to observe molecular distributions within intact separate cells effectively.
Taylor and his group show how this technique overcomes analytical challenges posed by organic samples and figuratively “breaks down the fluorescent curtain” in them for laser Raman microspectroscopy interrogation. Using this technique, they will trace cellular assimilation of isotopic tracers, document intracellular biochemical adjustments, and analyze various environmental units.
The investigators thus far have used the technique to investigate many cellular conditions, reminiscent of examining cell-to-cell abnormalities in growth charges of phytoplankton (microalgae), observing viral infections inside phytoplankton cells, tracing movements of vitamins from marine bacteria into microbial predators, and figuring out and quantifying microplastic particles in oceanic plankton samples.